The Nigeria Football Federation has set a deadline of March 20 for players to report to the Super eagles camp in London ahead of the friendly matches.
Nigeria will face Senegal on March 23, followed four days later by a clash against Burkina Faso.
Both matches will be played at the Hive Stadium in London.
Of the 25 players called up by Rohr for the March matches, no less than seven are based in England, including the likes of Alex Iwobi, Kelechi Iheanacho and Victor Moses.
Nigeria are using the matches to prepare for the 2019 Africa Cup of Nations qualifier against South Africa in June.
